5/18/2023 0 Comments Verified Kill by Patricia Logan![]() 81–176 mutants defective in autoagglutination showed a modest reduction in adherence and invasion of INT407 cells. This suggests that interactions between modifications on adjacent flagella filaments are required for autoagglutination. jejuni 81–176 and Campylobacter coli VC167 that produced flagella lacking PseAm or PseAc failed to autoagglutinate. ![]() Mutation of a second gene also resulted in loss of PseAm, as well as a minor modification that appears to be PseAm extended with N-acetyl-glutamic acid. Mutation of Cj1316c or pseA had been shown to result in loss of the acetamidino form of pseudaminic acid (PseAm). Mutation of 6 new genes, in addition to three previously reported, resulted in a non-motile phenotype, consistent with a role in synthesis of pseudaminic acid (PseAc) or transfer of PseAc to flagellin. Twenty-four of the 45 genes found between Cj1293 and Cj1337 in NCTC 11168 are missing in 81–176. ![]() Analysis of the complete flagellin glycosylation locus of Campylobacter jejuni strain 81–176 revealed a less complex genomic organization than the corresponding region in the genome strain, C. ![]()
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